Microscope Interface application examples

MeTiS: Characterization of inclusion population

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MeTiS : software application for automatic characterization of inclusion population in the metallurgical industry

MeTiS was developed by Datamet to characterize inclusion population automatically from images from an optical microscope or a system consisting of a scanning electron microscope and EDS analyzer.

Version for electron microscopy

Integrated FEG-SEM JEOL, image analysis (based on Aphelion™) and chemical analysis (EDS PGT) to detect and analyze the inclusions in the best conditions. Metis is a complete software control of the microscope and image analysis including three separate modules:

  • Metis to measure the morphological parameters and chemical
  • meTis processing and formatting results
  • metiS for the simulation of measurement methods

Simple and user friendly interface assists MeTiS logical user sessions setting during measurement, methods of analysis and simulation, while allowing to obtain a large number of possibilities.

meTis and metiS are hardware-independent analysis (FEG-SEM and EDS). They can be used on items deported.

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Metis (measure)

A strong coupling between the FEG-SEM (JEOL JSM-6500F), the EDS analysis system and software image analysis (Aphelion) allows an optimal characterization of inclusions with diameters ranging from a few hundred nanometers to a few tens micrometers.

The main specificities of Metis are:
  • number of samples only limited by the size of the plate and the sample size
  • no limit on the number of measurement locations on each sample
  • possible measures on samples rectangular or circular
  • possibility of linking characterizations with different measurement conditions
  • no limit on the duration of measurement
  • choice of type and image acquisition conditions
  • various methods for scanning the measurement zone
  • magnification change possible during the measurement session
  • autofocus each field
  • distinction to three phases of the matrix by thresholding
  • choice of chemical elements to analyze the different phases
  • choice of analytical conditions (position of the chemical analysis, duration)
  • backup images of inclusions or fields demand
  • choice among different measurement options (image processing, filters, etc.).
  • possibility of release in semi-automatic mode (manual focus and / or manual thresholding…)
  • automatic backup results
  • work session defined by the user credentials, test, sample, etc.

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The benefits of Metis Measure are:
  • automatic characterization of inclusions having a diameter greater than 500 nm
  • characterization inclusions smaller in semi-automatic mode
  • implementation quick and easy data
  • complete parameterization methods to multiply
  • observation and measurements at different magnifications (optimization of analysis time…)
  • measurements of the parameters of chemical and morphological many inclusions (up to more than 100/hour)
  • ability to analyze multiple samples with different measurement conditions
  • optimizing the use of the microscope (24&nsbsp;H/24 operation possible)
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Examples of inclusions detected

MeTis (Processing)

Processing software, dissociated measurement software allows multiple operations data measurement files, and return the results in Microsoft Excel.

The main specificities of MeTis are:
  • possibility of different treatments on each measurement file
  • treatment of results files (merge, filtering, etc.)
  • creation of tables of results
  • creation of multiple graphs (mapping, histogram, etc.)
  • automatic recognition of inclusion population
  • processing database images inclusions
  • constitution report results (specific methods or standards)
  • functions “macros”

The benefits of MeTis Treatment are :
  • treatment independent of the measurement
  • a multiprocessor database
  • expert system to identify inclusion population
  • automatic reports with ASTM E45 (2005), DIN 50602 (1985), ISO 4967 (1998), JIS G 0555 (2003) et EN 10247 (2007)
metiS (simulation)

The simulation software provides access to statistical parameters of the various methods by measures calculated on virtual samples.

The advantages of MetiS Simulation are :
  • uncertainty calculation for given measurement method
  • comparison of different methods
  • determination of optimal measurement conditions

Download article

An article describing the application is published in the journal JEOL News in July 2006.

Version for optical microscopy

MeTiS version for optical microscopy offers the same features as the version for electron microscopy, with the exception of the following features:

  • characterization inclusions smaller than 0.5 microns
  • chemical analysis and processing of data from this analysis
  • magnification change during a session

Grain boundary detection

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Grain boundary detection example

This software solves a common problem in materials science, namely the detection of grain boundaries. For example, ASTM standards refer to this detection.

The user interface appears as a combination of several windows with buttons calling some functions of image processing and mathematical morphology. The judicious combination of these features can automatically detect the grains and their boundaries in calling functions segmentation and analysis of size and shape. The user has very few parameters to be specified before the launch detection and the output image of the algorithm contains boundaries thinned and thick one pixel.

This software is absolutely expectations metallurgists who wish to automate the detection of grain material and quantify the number of phases and inclusions in steel. The software is based on a stand-alone program that takes an image in TIFF format as input and generates an image at the same output format. All measurements are exported to a file in Microsoft Excel format. The demo version is an executable Visual Basic.

The advantages of the detection software grain boundaries are:

  • Standalone software can be used from any Windows PC
  • Solves the problem non-trivial detection prior to any extent ASTM
  • No learning curve - Can be used by non expert technician in image processing

  • Automatic 99%. Very few parameters must be specified by the user
  • Fully compatible with all hardware supported by the acquisition of Aphelion™, as an optical microscope or digital microscope
  • Outputs a binary image and measures exported into a spreadsheet


The algorithm includes edge detection, two types of transformations Top Hat Form to solve the problem of non-uniform illumination, an edge detection, filtering, operations erosion and dilation to clean contours, a line algorithm Watershed and skeleton by influence zone.