Automatic Quantification for Scratch Wound Healing Assay

ADCIS developed a very innovative software application to automatically quantify wound healing over time. In this application, the quantification is efficiently performed in a more accurate, reliable, and reproducible manner.

Wound Healing Study - Role of HA molecular

This application is applied to evaluate the role of HA molecular weight in the proliferative phase of wound healing (Hyaluronic Acid).

Protocol

Scratch wound assay: Monolayer is wounded by a manual scratch with a pipette tip. Solutions containing different HA at 0.2% in culture medium with 2.5% of FCS are distributed at Day 0 and cultures are kept at 37 Celsius degrees for 24 hours (Day 1). Cells are placed in a Petri dish to study their evolution in time.

Results

The goal of the study is to compare the healing wound over time in function of the HA molecular weight. A comparison between high, medium, and low molecular weights is automatically performed.

Scratch wound assay depending on the molecular weight Hyaluronan (MW-HA)

Automatic computation of the healing surface area using image processing & analysis techniques

Measurement Analysis and conclusion

After 24 hours, medium molecular weight of Hyaluronan (MMW-HA) has highly increased cell proliferation compared to the control Petri dish that has a mono-layer cell population with no hyaluronic acid. Image processing helped to automatically extract the healing area and compute its surface area. This method is more robust and more accurate than a manual drawing, and also much faster.

Reference

Results provided by this software product were used by the research team who published the article referenced below:

  • Ghazi K, Deng-Pichon U, Warnet J-M, Rat P (2012) Hyaluronan Fragments Improve Wound Healing on In Vitro Cutaneous Model through P2X7 Purinoreceptor Basal Activation: Role of Molecular Weight. PLoS ONE 7(11): e48351. doi:10.1371/journal.pone.0048351