Ploidics: DNA Quantization and Ploidy Analysis

Download the flyer Contact us for sales information Related video

Segmentation results for one image of an image sequence, with Image Gallery shown

Typical ploidy histogram

Ploidy analysis is a test performed on cells using either an optical microscope hooked to a computer or a flow cytometer. It is based on the fact that tumors with a diploid pattern of DNA amounts tend to be benign. On the other hand, tumors with highly variable DNA patterns tend be more malignant.

The Ploidics™ product is a stand-alone, software product that performs image processing and image analysis on digital images captured with an optical microscope and stored on a computer. It also processes virtual slide images of any size and depth. Ploidics is an automatic tool to measure abnormal DNA content in individual cells in a mixed population of cells. It includes a fully automatic, powerful, and innovative segmentation process, an editing tool to manually annotate the segmentation result if desired, and an automatic computation of a histogram showing the DNA mass distribution of the cell population.

Capabilities

The Ploidics software product has the following capabilities:

  • Automatic splitting of touching nuclei
  • User selection of nuclei size used in the analysis
  • User decision to keep or discard heterogeneous nuclei
  • User specification of color band to be processed based on the stain selected
  • User modification of segmentation results to select/deselect cells used in the Ploidy analysis

Technical Specifications

Ploidics is a stand-alone, software product that can be installed on any personal computer running Windows® XP or higher. Images need to be accessible from a hard drive or from network storage, and organized in folders to be processed by the software. All Ploidics measurements are calibrated and output in real-world units. The calibration value is manually entered in the Ploidics Software as a single value.

Video Tutorial